In vitro toxicity test of 2-cyanoacrylate polymers by cell culture method

✍ Scribed by Tseng, Yin-Chao ;Tabata, Yasuhiko ;Hyon, Suong-Hyu ;Ikada, Yoshito

• Publisher: John Wiley and Sons
• Year: 1990
• Tongue: English
• Weight: 637 KB
• Volume: 24
• Category: Article
• ISSN: 0021-9304
• DOI: 10.1002/jbm.820241007

✦ Synopsis

The inhibition of Swiss 3T3 cell growth by the microspheres prepared from various 2-cyanoacrylate polymers was investigated to assess their cell toxicity. Poly(ethoxyethyl 2-cyanoacrylate) and poly(methy1 2-cyanoacrylate) microspheres inhibited cell growth in a smaller amount than poly-(isobutyl 2-cyanoacrylate) and poly(ethy1 2-cyanoacrylate) microspheres. The extent of cell growth inhibition by the microspheres decreased with the increasing molecular weight, regardless of the kind of polymers used. Every kind of the micro-spheres was degraded releasing formaldehyde in the culture medium. The cell growth inhibition by the medium containing the microspheres was observed within 24 h for poly(ethoxyethy1 2-cyanoacrylate) and poly(methy1 2-cyanoacrylate). The extent of inhibition was in a linear proportion with the amount of formaldehyde released. It is concluded that the cell toxicity of 2-cyanoacrylate polymers is attributed to formaldehyde released upon polymer degradation.