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Specific anti-tumor responses by cultured immune spleen cells. I. In vitro culture method and initial characterization of factors which block immune cell-mediated cytotoxicity in vitro

✍ Scribed by Karen Nelson; Sylvia B. Pollack; Karl Erik Hellström

Publisher
John Wiley and Sons
Year
1975
Tongue
French
Weight
642 KB
Volume
15
Category
Article
ISSN
0020-7136

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✦ Synopsis

Abstract

Spleen cells from BALB/c mice which either bore syngeneic sarcomas or were normal controls were cultured in vitro. The culture supernatants of spleen cells from tumor‐bearing mice inhibited (blocked) specific cell‐mediated cytotoxicity to the tumor borne by the spleen donor. They also mediated specific antiserum‐dependent cytotoxicity with control lymphoid cells. The appearance of blocking activity in culture supernatants was prevented by lysis of Q‐positive spleen cells with antiserum and complement. The blocking activity was removed by passing culture supernatants through an anti‐mouse immunoglobulin affinity column and was recovered in a 3 M NaSCN eluate of the column.

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