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In vitro synthesis and secretion of ecdysteroids byDrosophila melanogaster ovaries

✍ Scribed by Rubenstein, Elaine C. ;Kelly, Thomas J. ;Schwartz, Margaret B. ;Woods, Charles W.


Publisher
John Wiley and Sons
Year
1982
Tongue
English
Weight
307 KB
Volume
223
Category
Article
ISSN
0022-104X

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✦ Synopsis


Abstract

The ovaries of adult Drosophila melanogaster were shown to produce and secrete ecdysteroids in vitro by an ecdysteroid radioimmunoassay. The major secretory products were determined by HPLC/RIA analysis to be ecdysone, 20‐hydroxyecdysone, and a highly polar fraction. Based on competitive binding studies, the secreted ecdysteroids contain 2–3 times more 20‐hydroxyecdysone than ecdysone.


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