In vitro synthesis and secretion of ecdysteroids byDrosophila melanogaster ovaries
✍ Scribed by Rubenstein, Elaine C. ;Kelly, Thomas J. ;Schwartz, Margaret B. ;Woods, Charles W.
- Publisher
- John Wiley and Sons
- Year
- 1982
- Tongue
- English
- Weight
- 307 KB
- Volume
- 223
- Category
- Article
- ISSN
- 0022-104X
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✦ Synopsis
Abstract
The ovaries of adult Drosophila melanogaster were shown to produce and secrete ecdysteroids in vitro by an ecdysteroid radioimmunoassay. The major secretory products were determined by HPLC/RIA analysis to be ecdysone, 20‐hydroxyecdysone, and a highly polar fraction. Based on competitive binding studies, the secreted ecdysteroids contain 2–3 times more 20‐hydroxyecdysone than ecdysone.
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