Stimulatory effect of fractions of brain homogenate on protein synthesis in ovaries ofEisenia fetida Sav. In Vitro (Oligochaeta, Annelida)
✍ Scribed by Lattaud, C. ;Marcel, R.
- Publisher
- John Wiley and Sons
- Year
- 1993
- Tongue
- English
- Weight
- 471 KB
- Volume
- 266
- Category
- Article
- ISSN
- 0022-104X
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✦ Synopsis
Abstract
In Eisenia fetida, during the period of genital activity, the synthesis of androgen by testicular tissues is under the control of a neurohormone elaborated by cerebral ganglia (CG). These ganglia also have a trophic effect on ovarian protein synthesis. Present results confirm that incorporation in vitro of [^3^H]‐leucine by ovaries is stimulated by a cerebral neurohormone. Furthermore, fractions of brain homogenate, obtained by gel filtration on Sephadex G 25, have the same action on ovaries in organ culture as CG. Two specific fractions (fractions 3 and 4) stimulate protein synthesis in the ovary. In the ovarian oocytes, the rate of vitellogenesis is low, since in cultured isolated ovaries the amount of radioactive precursor incorporated is only 0.06% of the amount added and this percentage remains low in the ovaries cultured with fractions 3 and 4 (0.15% and 0.21%, respectively) or with CG (0.24%). Further research is necessary to obtain and characterize the biologically active material. © 1993 Wiley‐Liss, Inc.