In vitro studies of histone glycation
β Scribed by Bellis, Dominic De; Horowitz, Martin I.
- Book ID
- 122508446
- Publisher
- Elsevier Science
- Year
- 1987
- Tongue
- English
- Weight
- 281 KB
- Volume
- 926
- Category
- Article
- ISSN
- 0304-4165
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## Abstract Purified histones in solution, purified nuclei, or whole endothelial cells in cell culture were used to study the reactivity of histones with various sugars. The sugar incubation of purified histones produced nonenzymatic glycation and formation of histone crossβlinks showing disappeara
2-(ZFuroyl)-~2-furanyl)-l~-imidazole (FFI) is detected in total histones and the lysine-rich histone 1 fraction isolated from calf thymus after in vitro glycation and hydrolysis with HCl, using gas chromatogaphic/mass spectrometric analysis. The finding suggests that glucose is bound to the &-amino