In vitro Stimulation by Insulin of α-Amino-isobutyric Acid Transport in the Absence of Protein Synthesis
✍ Scribed by FRITZ, G. R.; KNOBIL, E.
- Book ID
- 109631720
- Publisher
- Nature Publishing Group
- Year
- 1963
- Tongue
- English
- Weight
- 124 KB
- Volume
- 200
- Category
- Article
- ISSN
- 0028-0836
- DOI
- 10.1038/200682a0
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## Abstract The synthesis of the leucine‐specific and LIV‐binding proteins was examined in vitro in a coupled transcription/translation system using the hybrid plasmids pOX7 and pOX13 as templates. Plasmid pOX7 contains the __livK__ gene coding for the leucine‐specific binding protein, and pOX13 co
A quantitative in vitro amino acid incorporation assay is described which can be used to assess the status of in vivo protein synthesis. The preparation and incubation conditions employed result in constant precursor specific activity and limit amino acid incorporation to completion of nascent pepti