In vitro properties of crosslinked, reconstituted collagen sheets

✍ Scribed by Morykwas, Michael J.

• Publisher: John Wiley and Sons
• Year: 1990
• Tongue: English
• Weight: 363 KB
• Volume: 24
• Category: Article
• ISSN: 0021-9304
• DOI: 10.1002/jbm.820240812

✦ Synopsis

Abstract

Reconstituted, 100‐μm‐thick collagen sheets were crosslinked with either UV light, chromium, or cysteine for use as a burn covering. The sheets were also exposed to a “surface agent” (hydroxyproline, fibronectin, or soluble basement membrane matrix containing Type IV collagen) as a preliminary step in planned adherence studies. Since some chemicals render the collagen toxic, the modified sheets were tested for cytotoxicity using human keratinocytes and fibroblasts. Autoradiography and ^3^H‐thymidine incorporation were used to quantitate the proliferative rate of these cells in vitro. There was a universal depression of keratinocyte incorporation of ^3^H‐thymidine following a 1‐day exposure to any collagen sheet when compared to cells not exposed to any collagen. This effect had lessened by 5 days' exposure to the collagen. Conversely, the fibroblasts showed an enhancement in rate of incorporation after 1‐day exposure, especially for cells exposed to collagen sheets crosslinked by UV light. This effect had also lessened by 5 days' exposure. Autoradiography showed few significant variations for any of the cells exposed for either time period. Chromium leaching was determined, with no values greater than 30% of the allowable maximum set by both the British and American Pharmacopeia.