Three culture types of Hypoxis rooperi T. Moore were examined to determine whether hypoxoside was present. Of these cultures, only root-type cultures were found to contain hypoxoside. Quantification of this compound within these tissues using HPLC, indicated that malformed root (MR) cultures contain
In vitro propagation ofHypoxis rooperi
β Scribed by Y. M. Page; J. Staden
- Book ID
- 104620386
- Publisher
- Springer
- Year
- 1984
- Tongue
- English
- Weight
- 346 KB
- Volume
- 3
- Category
- Article
- ISSN
- 0167-6857
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β¦ Synopsis
The propagation of Hypoxis rooperi S. Moore in vitro, was most efficiently achieved by initially culturing corm explants on a basal medium (BM) supplemented with 1.0 mg 1-J 6-benzylaminopurine (BA). Explants which developed shoots only on this medium, could be rooted when transferred onto a hormone-free BM. Utilizing this technique, an average of 39 plants could be obtained from a mature corm, 90% of which could be established in a sandy soil.
π SIMILAR VOLUMES
The effect of various concentrations of benzyladenine or kinetin (4.7-185.9 ~tM) on shoot proliferation from shoot-tip explants was investigated in C. praetermissum Heiser & Smith and C. annuum L. Maximum number of shoots were obtained on Murashige & Skoog's medium with 66.6 ~tM BA or 92.9 ~tM kinet
Multiple shoot formation and their elongation from excised apical vegetative shoots of a 40-year old-tree of Mitragyna parvifolia Korth. was achieved in Murashige and Skoog's medium supplemented with 4.44 pM benzyl adenine. The in vitro regenerated shoots rooted when cultured on modified Murashige a