๐”– Bobbio Scriptorium
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In vitro clonal propagation ofCapsicumspp.

โœ Scribed by T. Christopher; M. V. Rajam


Book ID
104616086
Publisher
Springer
Year
1994
Tongue
English
Weight
359 KB
Volume
38
Category
Article
ISSN
0167-6857

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โœฆ Synopsis


The effect of various concentrations of benzyladenine or kinetin (4.7-185.9 ~tM) on shoot proliferation from shoot-tip explants was investigated in C. praetermissum Heiser & Smith and C. annuum L. Maximum number of shoots were obtained on Murashige & Skoog's medium with 66.6 ~tM BA or 92.9 ~tM kinetin in C. praetermissum, and 88.8 ~tM BA or 116.2 ~tM kinetin in C. annuum after 4 weeks of culture. Combining 1 ~M 2, 3, 5-triiodobenzoic acid (TIBA) with low levels of BA or kinetin significantly increased shoot number as compared to using either cytokinin alone. Rooting of regenerated shoots was achieved on MS medium containing 5.7 ~tM indoleacetic acid. Best rooting (80-100%) was observed in shoots from TIBA plus BA or kinetin media while only 40-50% of shoots from the BA or kinetin treatments were rootable. Plantlets obtained from TIBA plus BA or kinetin were normal diploids while those from BA or kinetin alone revealed distinct chromosomal aberrations in their root tip squashes. Regenerants from TIBA plus BA or kinetin media were successfully established in the soil (86% survival rate), where they flowered and showed normal meiotic behaviour with 100% pollen viability.


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