In vitro clonal propagation of dioeciousCarica papaya

✍ Scribed by O. Reuveni; D. R. Shlesinger; U. Lavi

• Book ID: 104616344
• Publisher: Springer
• Year: 1990
• Tongue: English
• Weight: 445 KB
• Volume: 20
• Category: Article
• ISSN: 0167-6857
• DOI: 10.1007/bf00034755

✦ Synopsis

A procedure for in vitro propagation of dioecious papaya clones is described. A high rate of success in culture estbalishment was obtained when axillary buds were taken from lateral shoots of hedged rooted cuttings grown in a greenhouse. Seasonal endophytic contamination was suppressed by shaking propagules for 24 h in 300 mg 1-] rifampicin or by incorporating it at 50 mg 1-1 into the medium. Murashige & Skoog (MS) basal medium supplemented with 0.5 mgl 1 6-benzyladenine and 0.1 mg I 1 naphthaleneacetic acid was used for establishment and proliferation. The addition of 160 mg 1-1 adenine sulfate improved multiplication and shoot growth. An elongation stage on MS medium supplemented with 1.0mg1-1 kinetin and 0.05 mgl-J naphthaleneacetic acid was necessary before rooting. Rooting was obtained at a high rate on half-strength macroelements of MS medium supplemented with 1.0mgl i indole-3-butyric acid. Commercial plots of papaya plants obtained through this procedure already exist.