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In vitro propagation of cassava (Manihot esculentaCrantz)

✍ Scribed by Michael K. Smith; Brenda J. Biggs; Kenneth J. Scott

Publisher
Springer
Year
1986
Tongue
English
Weight
899 KB
Volume
6
Category
Article
ISSN
0167-6857

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✦ Synopsis

A method is presented for the rapid in vitro propagation of cassava (Manihot esculenta Crantz). Nodal explants were induced to grow as multiple-shoot cultures on a medium containing 1.0/zM 6-benzylamino purine (BAP), supplemented with 0.25/~M anaphthateneacetic acid (NAA). Nodes were removed from the shoots after three weeks of growth and subcultured on fresh culture medium. An average of 7.0 nodes were produced from each explanted node after three weeks in culture. Nodal explants were transferred to a medium containing 2.5/~M indole-3-butyric acid (IBA) to improve root initiation on the developing plantlets. Plant establishment was possible upon transfer to soil. In vitro propagation offers enhanced rates of multiplication over more conventional methods of propagation. In addition, in vitro propagation facilitates the storage and international exchange of cassava germplasm.

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