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Evaluation of cassava (Manihot esculentaCrantz) germplasm collections using RAPD markers

✍ Scribed by Philippe Marmey; John R. Beeching; Serge Hamon; André Charrier

Publisher: Springer
Year: 1994
Tongue: English
Weight: 642 KB
Volume: 74-74
Category: Article
ISSN: 0014-2336
DOI: 10.1007/bf00040402

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✦ Synopsis

Randomly amplified polymorphic DNA (RAPD) markers combined with the Simple Matching index for calculating the distance matrix were used to determine the genetic diversity of African cassava clones. A preliminary study of three Manihot species using 20 primers clearly showed that RAPD markers were relevant for analyzing their genetic diversity. DNAs from 19 cultivars of Manihot esculenta Crantz (cassava) were amplified using 8 primers. Cultivars were well discriminated and coherently distributed, comparing to previous results from isozymes and RFLP studies. Genetic diversity analysis, characterization of collections and study of introgression are the domains that RAPD markers can contribute to the improvement of cassava.

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