In vitro microwave effects on human neutrophil precursor cells (CFU-C)

✍ Scribed by Mark J. Ottenbreit; Susumu Inoue; Ward D. Peterson Jr.; James C. Lin

• Publisher: John Wiley and Sons
• Year: 1981
• Tongue: English
• Weight: 663 KB
• Volume: 2
• Category: Article
• ISSN: 0197-8462
• DOI: 10.1002/bem.2250020302

✦ Synopsis

Abstract

Human marrow cells were irradiated with 2450‐MHz CW microwaves in a fluid‐filled waveguide irradiation system. Cell exposure was conducted by placing a marrow cell suspension in 20‐μl glass microcapillary tubes that were positioned in the exposure chamber, and irradiated at power densities from 31 to 1,000 mW/cm^2^ (with corresponding specific absorption rates of 62 to 2,000 mW/g) for 15 minutes. The temperature of the sample was maintained at a fixed point. Sham‐irradiated (SI) and microwave‐irradiated (MWI) cells were cultured in a methylcellulose culture system for neutrophil colony proliferation. There was no reduction in neutrophil colony number on days 6–7 or 12–14 in cells exposed at 31 or 62 mW/cm^2^, but as the power density was increased to 1,000 mW/cm^2^, there was a reduction in colony number of MWI cells compared with SI cells. The microwave interaction with the human neutrophil colony‐forming cells was apparently not related to temperature rise, or to the state of cell cycle, and was irreversible.