In vitro effect of ascorbic acid on neutrophil-endothelial cell interaction

✍ Scribed by Jonas, Eleonore ;Dwenger, Alexander ;Hager, Anke

• Publisher: John Wiley and Sons
• Year: 1993
• Weight: 382 KB
• Volume: 8
• Category: Article
• ISSN: 0884-3996
• DOI: 10.1002/bio.1170080104

✦ Synopsis

The effect of different concentrations (0.06, 0.6 and 6.0 mmol/L) of ascorbic acid on neutrophil-endothelial interaction was studied using an in virro model of human umbilical cord vein endothelial cells and human neutrophils. The aim of the study was t o determine changes in chemiluminescence response of neutrophils during adherence t o endothelial cells. Because adherence of neutrophils t o endothelial cells is an essential component in inflammatory processes leading t o endothelial cell injury, the influence of ascorbic acid on adherence and endothelial cell injury have been investigated. Production of oxygen-derived metabolites, measured by chemiluminescence response of neutrophils, decreased significantly in the presence of 6 mmol/L ascorbic acid during coincubation of neutrophils and endothelial cells (p < 0.025). The adherence of neutrophils t o endothelial cells was significantly decreased at a concentration of 6 mmol/L (p < 0.0005). The inhibition of neutrophil adherence t o endothelial cells was correlated w i t h a diminished neutrophil-mediated endothelial cell injury during incubation w i t h 6 mmol/L ascorbic acid (p < 0.0005). The present results indicate that ascorbic acid might exert a protective effect on neutrophil-mediated endothelial cell injury by decreasing adherence of neutrophils t o endothelial cells and by scavenging reactive oxygen metabolites. Moreover, the current investigation points t o probable protective effect of ascorbic acid on oxidant-mediated cell damage in diseases (e.g., Adult Respiratory Distress Syndrome).