In vitro functional assay of alleles and haplotypes of two COL1A1-promoter SNPs
✍ Scribed by Natàlia García-Giralt; Anna Enjuanes; Mariona Bustamante; Leonardo Mellibovsky; Xavier Nogués; Ramón Carreras; Adolfo Díez-Pérez; Daniel Grinberg; Susana Balcells
- Book ID
- 116319112
- Publisher
- Elsevier Science
- Year
- 2005
- Tongue
- English
- Weight
- 409 KB
- Volume
- 36
- Category
- Article
- ISSN
- 8756-3282
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We have analyzed the function of the CYP1A1 promoter using in vitro transcription. Using nuclear extracts from HeLa cells, we found that transcription from the promoter in vitro was constitutive. Transcription in vitro was not increased by prior exposure of the HeLa cells to the inducer 2,3,7, 8-tet