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Analysis of CYP1A1 promoter function by transcription in vitro

✍ Scribed by Long-Ping Wen; Keith W. Jones; James P. Whitlock Jr.

Publisher: John Wiley and Sons
Year: 1991
Tongue: English
Weight: 363 KB
Volume: 4
Category: Article
ISSN: 0899-1987
DOI: 10.1002/mc.2940040203

No coin nor oath required. For personal study only.

✦ Synopsis

We have analyzed the function of the CYP1A1 promoter using in vitro transcription. Using nuclear extracts from HeLa cells, we found that transcription from the promoter in vitro was constitutive. Transcription in vitro was not increased by prior exposure of the HeLa cells to the inducer 2,3,7, 8-tetrachlorodibenzo-p-dioxin nor by the inclusion of a dioxin-responsive enhancer in the DNA template. Analyses of mutants revealed that a TATAAA sequence and two recognition motifs for CCAAT box transcription factor/nuclear factor I contributed to the constitutive activity of the promoter in vitro.

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