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In vitro fertilization of rabbit ova

✍ Scribed by Brackett, Benjamin G. ;Williams, William L.

Publisher: John Wiley and Sons
Year: 1965
Tongue: English
Weight: 953 KB
Volume: 160
Category: Article
ISSN: 0022-104X
DOI: 10.1002/jez.1401600305

No coin nor oath required. For personal study only.

✦ Synopsis

A repeatable procedure for in uitl-o fertilization of rabbit ova in which the percentage of ova fertilized is consistently high has been developed. When ova were recovered and subsequently incubated i n viho with capacitated spermatozoa at 37" or 39°C levels of ova fertilized were 15, 24 and 28% when 73-78%, 83%, and 90% relative humidities, respectively, were maintained. Twenty-one per cent of all ova in these experiments were fertilized. When the ova were recovered at 30-39°C and subsequently incubated at 37" or 39°C with capacitated spermatozoa under paraffin oil only 19% of the ova were fertilized. However, when ova were recovered and subsequently incubated with capacitated spermatozoa and fluids from the female

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