## Abstract After a 1βhour preincubation of epididymal mouse spermatozoa at a concentration of 2 Γ 10^6^/ml in 0.23 mM spermine, the proportion of F~1~ (C57BL Γ CBA) mouse ova fertilized after 1 and 2 hours was significantly greater than with untreated spermatozoa. Spermine also significantly incre
In vitro fertilization (IVF) of mouse ova in HEPES-buffered culture media
β Scribed by Barry R. Behr; C. J. Stratton; W. D. Foote; V. Knutzen; G. Sher
- Publisher
- Springer US
- Year
- 1990
- Tongue
- English
- Weight
- 878 KB
- Volume
- 7
- Category
- Article
- ISSN
- 1573-7330
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Epididymal sperm were collected from C57B16/J x DBA2/J (B6D2) males and allowed to capacitate for 2 hr. When cumulus-free oocytes were exposed to sperm for 15 min in either the presence (6.0 mM) or absence of caffeine, fertilization did not occur. However, when cumulus cells were left intact, 23% of
## Abstract Mouse ova with or without follicular cells (denuded) were treated with wheat germ agglutinin (WGA) in concentrations ranging from 5β500 ΞΌg/ml and fertilized in vitro. Ova subjected to concentrations greater than 10 ΞΌg/ml WGA were incapable of fertilization in vitro, although sperm were