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Effects of wheat germ agglutinin on fertilization of mouse ova in vivo and in vitro

โœ Scribed by Parkening, Terry A. ;Chang, M. C.

Publisher
John Wiley and Sons
Year
1976
Tongue
English
Weight
522 KB
Volume
195
Category
Article
ISSN
0022-104X

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โœฆ Synopsis

Abstract

Mouse ova with or without follicular cells (denuded) were treated with wheat germ agglutinin (WGA) in concentrations ranging from 5โ€“500 ฮผg/ml and fertilized in vitro. Ova subjected to concentrations greater than 10 ฮผg/ml WGA were incapable of fertilization in vitro, although sperm were observed adhering to the zona pellucida after treatment in concentrations as high as 500 ฮผg/ml WGA. At concentrations of 50 ฮผg/ml or higher, WGA adversely affected the shape of the vitellus. Of various saccharides employed to block the effects of WGA Dโ€glucose was the most effective.

Injections of 0.03 ml of 100 ฮผg/ml or 500 ฮผg/ml WGA into the ovarian bursa two to three hours before the expected time of ovulation had little or no effect on fertilization in vivo.

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