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In vitro fertilization in the sheep: Effect of elevated calcium concentration at insemination

✍ Scribed by Huneau, D. ;Crozet, N.

Book ID
111663463
Publisher
Wiley (John Wiley & Sons)
Year
1989
Weight
450 KB
Volume
23
Category
Article
ISSN
0148-7280

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## Abstract Rat epididymal spermatozoa, at a concentration of 40–65 Γ— 10^4^ cells/ml, were preincubated for 5–5.5 hr and then added to eggs from superovu‐lated, immature rats. By 10–12 hr after insemination, 86% of the eggs had undergone fertilization, but many of them (41%) were polyspermic. When