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In vitro fertilization and capacitation-like interaction in the hydroidCampanularia flexuosa

✍ Scribed by O'Rand, Michael G.

Publisher: John Wiley and Sons
Year: 1972
Tongue: English
Weight: 564 KB
Volume: 182
Category: Article
ISSN: 0022-104X
DOI: 10.1002/jez.1401820303

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✦ Synopsis

Fertilization in Campanularia jlexuosa is internal. In both freshly collected colonies and laboratory cultured colonies eggs may be removed from the gonangium still attached to the blastostyle as a single packet surrounded by epithelial cells. Packets from mature gonangia of freshly collected colonies contain spermatozoa although the eggs are immature. The spermatozoa remain inside the packet to await egg maturation. Packets from mature gonangia of laboratory cultured colonies do not contain spermatozoa if male and female colonies are cultured separately. Eggs in such packets may be fertilized by the addition of spermatozoa. Removal of the epithelial cells which surround the eggs in the egg packet results in loss of fertilizability. Treatment of the egg packets with 0.75% trypsin also results in a loss of fertilizability. However, fertilizability may be restored by exposure of spermatozoa to non-trypsin treated female epithelial cells. Thus, apparently a definite interaction must occur between female epithelial cells and spermatozoa prior to fertilization. This interaction may be analogous to mammalian capacitation and the necessary conditioning of amphibian sperm prior to fertilization.

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