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In vitro culture of female date palm (Phoenix dactyliferaL.) tissues

✍ Scribed by D. R. Sharma; Rita Kumari; J. B. Chowdhury

Publisher: Springer
Year: 1980
Tongue: English
Weight: 363 KB
Volume: 29
Category: Article
ISSN: 0014-2336
DOI: 10.1007/bf00037263

No coin nor oath required. For personal study only.

✦ Synopsis

Roots, leaf petioles, shoot tips and immature fruits of female date palm formed the source material for in vitro cultures. Isolated embryos excised from mature seeds were also cultured. Of all the tissues, some of the leaf petioles and fruit mesocarp gave rise to calluses in the media of SrArorsKv (1970) and EEUWENS (1976). Isolated embryos germinated to produce seedlings in MS (MURASHIGE & SKOOC, 1962) medium. Calluses were established from seedling sections but the growth conditions propitious for explants of seedlings were not suitable for female date palm tissues. The main problem in establishing long term tissue cultures has been the browning of the tissues and media. This problem was, at least partially, overcome by adding activated charcoal to the liquid media and cysteine to the solid madia. So far, shoot regeneration in the calluses could not be induced. 1NTRODUCTlON

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