In vitro plant regeneration and ethylmethanesulphonate (EMS) uptake in somatic embryos of date palm (Phoenix dactyliferaL.)

✍ Scribed by M. S. Omar; F. J. Novak

• Publisher: Springer
• Year: 1990
• Tongue: English
• Weight: 878 KB
• Volume: 20
• Category: Article
• ISSN: 0167-6857
• DOI: 10.1007/bf00041880

✦ Synopsis

The effect of the auxins dicamba (3,6-dichloro-2-methoxybenzoic acid) and picloram (4-amino-3,5,6-trichloropicolinic acid) on callus growth and embryogenesis in Phoenix dactylifera L. was investigated. Maximum callus fresh weight was obtained in nutrient medium enriched with 200 #m picloram. Somatic embryogenesis and subsequent plant regeneration was achieved following transfer of such calli to hormonefree medium. Germination of the somatic embryos was influenced by treatment with the chemical mutagen ethylmethanesulphonate (EMS). Uptake of the labelled mutagen ([14C]EMS) by the somatic embryos increased with increased incubation time. Presence of dimethyl sulphoxide (DMSO) as a carrier agent during mutagenic treatment was necessary for efficient mutagen uptake.