In vitro characterization of a recombinant32P-phosphorylated anti-(carcinoembryonic antigen) single-chain antibody
β Scribed by Mark R. Patrick; Kerry A. Chester; G. A. Pietersz
- Publisher
- Springer-Verlag
- Year
- 1998
- Tongue
- English
- Weight
- 375 KB
- Volume
- 46
- Category
- Article
- ISSN
- 0340-7004
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## Abstract In order to analyze the epitope structure of carcinoembryonic antigen (CEA) and the idiotype network system, seven antiβidiotypic monoclonal antibodies (antiβId MoAbs) were generated from a BALB/c mouse immunized with antiβCEA MoAb P1β356, which recognized a synthetic peptide P1 of CEA,
A bispecific monoclonal antibody (BsMAb) recognising carcinoembryonic antigen (CEA) and doxorubicin (Dox) was used in colorimetric microcytotoxicity assays with 3 human colon cancer cell lines (COLO320DM, SKCO1 and LS174T) showing no, high or medium CEA expression, respectively. The IC50 values for