We have set up a fast and easy methodology to identify cell-surface proteins in live yeasts. A non-gel proteomic approach was based on a short period of trypsin treatment followed by peptide separation and identification using nano-LC followed by off-line MS/MS. Candida albicans was used as a model
In vitro and in vivo cell surface expression of a thiol-specific antioxidant-like protein in Candida albicans
✍ Scribed by V. Apaire-Marchais; J. Cottin; A. Marot-Leblond; C. Lefrançois; G. Tronchin; R. Robert
- Publisher
- Masson Editeur
- Year
- 2005
- Tongue
- French
- Weight
- 650 KB
- Volume
- 15
- Category
- Article
- ISSN
- 1156-5233
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