Identification of Candida albicans exposed surface proteins in vivo by a rapid proteomic approach

✍ Scribed by María Luisa Hernáez; Pilar Ximénez-Embún; Montserrat Martínez-Gomariz; María Dolores Gutiérrez-Blázquez; César Nombela; Concha Gil

• Publisher: Elsevier
• Year: 2010
• Tongue: English
• Weight: 273 KB
• Volume: 73
• Category: Article
• ISSN: 1874-3919
• DOI: 10.1016/j.jprot.2010.02.008

✦ Synopsis

We have set up a fast and easy methodology to identify cell-surface proteins in live yeasts. A non-gel proteomic approach was based on a short period of trypsin treatment followed by peptide separation and identification using nano-LC followed by off-line MS/MS. Candida albicans was used as a model organism and proteins involved in cell wall organization, cell rescue, defense, virulence, transport, protein fate and metabolism were identified. This strategy is a powerful tool to study host-pathogen interactions and to look for potential vaccine candidates and drug targets.