In vitro and in vivo biocompatibility studies of a recombinant analogue of spidroin 1 scaffolds

✍ Scribed by M. M. Moisenovich; O. L. Pustovalova; A. Yu Arhipova; T. V. Vasiljeva; O. S. Sokolova; V. G. Bogush; V. G. Debabov; V. I. Sevastianov; M. P. Kirpichnikov; I. I. Agapov

• Publisher: John Wiley and Sons
• Year: 2010
• Tongue: English
• Weight: 544 KB
• Volume: 96A
• Category: Article
• ISSN: 1549-3296
• DOI: 10.1002/jbm.a.32968

✦ Synopsis

Abstract

The goal of this study was to generate porous scaffolds from the genetically engineered protein, an analogue of Nephila clavipes spidroin 1 (rS1/9) and to assess the properties of new rS1/9 scaffolds essential for bioengineering. The salt leaching technique was used to make the rS1/9 scaffolds of interconnected macroporous structure with spontaneously formed micropores. The tensile strength of scaffolds was 18 ± 5 N/cm^2^. Scaffolds were relatively stable in a phosphate buffer but degraded in oxidizing environment after 11 weeks of incubation. Applicability of the recombinant spidroin 1 as a substrate for cell culture was demonstrated by successful 3T3 cells growth on the surface of rS1/9 films (270 ± 20 cells/mm^2^
vs. 97 ± 8 cells/mm^2^ on the glass surface, p < 0.01). The 3T3 fibroblasts readily proliferated within the rS1/9 scaffold (from initially plated 19 ± 2 cells/mm^3^ to 3800 ± 304 cells/mm^3^ after 2 weeks). By this time, cells were uniformly distributed between the surface and deeper layers (27% ± 8% and 33% ± 4%, respectively; p > 0.05), whereas the initial distribution was 58% ± 7% and 11% ± 8%, respectively; p < 0.05). The rS1/9 scaffolds implanted subcutaneously into Balb/c mice were well tolerated. Over a 2‐month period, the scaffolds promoted an ingrowth of de novo formed vascularized connective tissue elements and nerve fibers. Thus, scaffolds made of the novel recombinant spidroin 1 analogue are potentially applicable in tissue engineering. © 2010 Wiley Periodicals, Inc. J Biomed Mater Res Part A, 2010.