The expression of myelin proteins in actively myelinating quaking and control brains was studied. RNA was extracted from the brains of 18- and 27-day-old mice and analyzed by northern blot using cDNA probes for proteolipid protein (PLP), basic protein (BP), and myelin-associated glycoprotein (MAG).
In vitro acylation of myelin PLP and DM-20 in the quaking mouse brain
β Scribed by Harish C. Agrawal; Daya Agrawal; Takeo Yoshimura; Joyce A. Benjamins
- Book ID
- 105020851
- Publisher
- Springer
- Year
- 1987
- Tongue
- English
- Weight
- 555 KB
- Volume
- 12
- Category
- Article
- ISSN
- 0364-3190
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## Abstract The most common cause of PelizaeusβMerzbacher (PMD) is due to duplication of the __PLP1__ gene but it is unclear how increased gene dosage affects PLP turnover and causes dysmyelination. We have studied the dynamics of PLP/DM20 in a transgenic mouse model of PMD with increased gene dosa
Characteristics of fatty acylation of myelin proteolipid protein (PLP) in vitro were compared with the corresponding process in vivo. Rapid and efficient separation of labelled PLP from other proteins and lipids was effected by extraction into chloroform/methanol/ 0.1 N HCI (10/10/1) and chromatogra