𝔖 Bobbio Scriptorium
✦   LIBER   ✦

In vitro activation of the in vivo colony-forming units of the mouse yolk sac

✍ Scribed by Gershon Perah; Michael Feldman

Publisher
John Wiley and Sons
Year
1977
Tongue
English
Weight
545 KB
Volume
91
Category
Article
ISSN
0021-9541

No coin nor oath required. For personal study only.

✦ Synopsis

Abstract

Experiments were performed to investigate the presence of colony‐forming units (CFU) in the mouse embryonic yolk sac during the developmental period in which the yolk sac is the sole hemopoietic organ. Injection of yolk sac cell suspensions from normal embryos into syngeneic, lethally irradiated adult recipients evoked a very low number of spleen colonies. However, prior cultivation of yolk sacs in vitro caused a dramatic increase in the spleen colony‐forming capacity–as high as 84‐fold–following 48 hours in culture. The yolk sac origin of the spleen colonies was confirmed by: (a) Chromosomal marker analysis; (b) dose‐response analysis; (c) demonstrating that the above colonies were not of endogenous origin induced by the mere injection of grafted cells. We conclude that the yolk sac contains many precursors of colony‐forming cells which though undetectable by immediate grafting apparently become activated in culture by an as yet unknown induction process.

📜 SIMILAR VOLUMES

The ultrastructure of megakaryopoietic c
The ultrastructure of megakaryopoietic cells of the yolk sac and liver in mouse embryo
✍ Matsumura, George ;Sasaki, Kazunobu 📂 Article 📅 1988 🏛 John Wiley and Sons 🌐 English ⚖ 801 KB

Megakaryopoietic cells in the yolk sac and liver of mouse embryos were examined by electron microscopy. At 10 days' gestation, yolk sac vitelline vessels contained a few free megakaryopoietic cells. On the basis of the development of demarcation membranes and granules, yolk sac megakaryopoietic cell

Yolk-sac carcinoma of extra-embryonic or
Yolk-sac carcinoma of extra-embryonic origin in the 129 Sv/Sl mouse
✍ Halina Sobis; Luc Van Hove; Michel Vandeputte 📂 Article 📅 1983 🏛 John Wiley and Sons 🌐 French ⚖ 884 KB

## Abstract Displacement of the visceral yolk‐sac in fetectomized rats and hamsters does not only induce benign teratomas but may also lead to the development of malignant yolksac carcinoma. In the mouse, displacement of this extraembryonic membrane readily induces teratoma but until now yolk‐sac c

The detection of in vitro monocyte-macro
The detection of in vitro monocyte-macrophage colony-forming cells in mouse thymus and lymph nodes
✍ T. J. MacVittie; K. F. McCarthy 📂 Article 📅 1977 🏛 John Wiley and Sons 🌐 English ⚖ 311 KB

## Abstract In vitro moocyte‐macrophage colony‐forming cells (CFC) have been detected in the thymus (30/10^6^ cells) and in the cervical (22/10^6^) and mesenteric (20/10^6^) lymph nodes (LN) of the mouse. Thymus and LN derived CFC differed from bone marrow derived CFU‐c in several characteristic pa

Heterogeneity of in vitro colony- and cl
Heterogeneity of in vitro colony- and cluster-forming cells in the mouse marrow: Segregation by velocity sedimentation
✍ Dr. D. Metcalf; H. R. Macdonald 📂 Article 📅 1975 🏛 John Wiley and Sons 🌐 English ⚖ 796 KB

## Abstract C~57~BL bone marrow cells were separated on the basis of their sedimentation velocity at unit gravity and cell fractions cultured in agar using three types of colony stimulating factor (CSF). Colony‐forming cells separated as a single peak (s = 4.4 mm/hr) in cultures stimulated by mouse