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In SituMonitoring of Adsorption of Lysozyme onto Quartz Using an Electrode-Separated Piezoelectric Sensor

โœ Scribed by D.Z. Shen; Y.H. Xue; Q. Kang; Q.J. Xie; L.X. Chen

Publisher
Elsevier Science
Year
1998
Tongue
English
Weight
80 KB
Volume
60
Category
Article
ISSN
0026-265X

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โœฆ Synopsis

The adsorption of lysozyme from phosphate-buffered saline (pH 7.0) onto a quartz crystal interface was investigated in situ using an electrode-separated piezoelectric sensor (ESPS). Increasing amounts of lysozyme adsorbed onto a quartz crystal surface resulted in linearly decreasing oscillating frequencies of the ESPS. The lysozyme adsorbed is partially desorbed after rinsing with the phosphate-buffered saline. The amounts adsorbed reversibly and irreversibly were estimated. The influence of the surface roughness of the quartz crystal on the adsorption density measurement is discussed.

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