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In situfiltration ofAnchusa officinalisculture in a cell-retention stirred tank bioreactor

✍ Scribed by Wei Wen Su

Publisher
Springer-Verlag
Year
1995
Tongue
English
Weight
376 KB
Volume
9
Category
Article
ISSN
0951-208X

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✦ Synopsis

The effect of agitation and aeration on filtration of nnchusu ofjcinalis culture in a stirred tank bioreactor integrated with an internal filter unit was investigated. Increases in suction head of the pump that drove the filtration process wcrc measured at impeller speeds cif 100 and 200 rpm. Surprisingly, suction head attained at 200 rpm was about 40% higher than at 100 rpm. Direct observation of the cake deposition process in the reactor using a dilute cell suspension rcvcaled that the filter cake formed at 100 rpm was thicker, but less compact. Aeration at 0.4 vvm was shown to have little effect on the filtration rate, since the bulk fluid flow was dominated by the impcllcr hydrodynamics. The initial flux can be recovered by filter backwashing with compressed air at a flow rate of 0.6 vvm for a duration of 5 minutes.

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