Photolithotrophic cultivation of Laminaria saccharina gametophyte cells in a stirred-tank bioreactor

✍ Scribed by Hans Qi; Gregory L. Rorrer

• Publisher: John Wiley and Sons
• Year: 1995
• Tongue: English
• Weight: 947 KB
• Volume: 45
• Category: Article
• ISSN: 0006-3592
• DOI: 10.1002/bit.260450310

✦ Synopsis

Filamentous cell cultures derived from female gametophytes of the temperate brown macroalga Laminaria saccharina were photolithotrophically cultivated in artificial seawater medium within an illuminated 1.3-L stirred-tank bioreactor at 13°C using CO, in air as the carbon source.

A Monod model adequately described light-saturated growth. The apparent half-saturation constant (KO) was 23 pE/m2-s, and maximum specific growth rate was 0.15 day-'. At a constant inoculation cell density of 50 mg DCWIL, biomass productivity after 26 days of cultivation increased from 630 mg DCW/L at 18 kE/m2-s to 890 mg DCW/L at 228 pE/m2-s. At 98 pE/mZ-s, 1.1 vvm aeration rate, and 250 rpm impeller speed, the CO, transfer rates (CO, TRs) and CO, consumption rates (rco were determined over the cultivation period. At peak k0, demand, the maximum CO, TR was 0.19 mmol CO,/L-h, but rco, was only 0.15 mmol CO,/L-h, implying that the culture was not CO, transport limited. This is the first reported bioreactor cultivation study of cell cultures derived from a macrophytic marine alga.