In situ N-phosphorylation of oligopeptides for fast atom bombardment mass spectrometry

✍ Scribed by Hou-Jun Yang; Mei-Yu He; Yun-Hua Ye; Yu-Fen Zhao

• Publisher: John Wiley and Sons
• Year: 1992
• Tongue: English
• Weight: 341 KB
• Volume: 27
• Category: Article
• ISSN: 1076-5174
• DOI: 10.1002/oms.1210270618

✦ Synopsis

Positive ion fast atom bombardment mass spectrometry (FABMS) of in situ N-phosphorylated oligopeptides showed intense quasi-molecular ions together with the successive alkene loss fragment ions, which afford multiple checks of the unequivocal reality of the relative molecular mass of the tested samples More interesting, in a novel cleavage pattern only tbe N-pbosphoryl fragment ioas gave intense peaks, the C-terminal series ions being s u p pressed. For each of the N-terminal ions, losses of alkenes also occur to provide multiple cbecks for the existence of tbese ions. The FABMS of the in situ N-phospborylated oligopeptides might provide an easily accessible routine method for peptide sequencing.