In situ analysis of breast cancer progression in murine models using a macroscopic fluorescence imaging system

✍ Scribed by Alicia L. Carlson; Michaela R. Hoffmeyer; Kristin M. Wall; Paige J. Baugher; Rebecca Richards-Kortum; Suranganie F. Dharmawardhane

• Publisher: John Wiley and Sons
• Year: 2006
• Tongue: English
• Weight: 333 KB
• Volume: 38
• Category: Article
• ISSN: 0196-8092
• DOI: 10.1002/lsm.20409

✦ Synopsis

Background and objective:

The goal of this study was to use an inexpensive macroscopic imaging system to monitor tumor progression in mouse models in real-time with minimal intervention.

Study design/materials and methods:

Illumination is provided via a xenon arc lamp and a fiber optic probe which delivers white light or quasi-monochromatic excitation via specific bandpass filters. fluorescence emission from scid and nude mice following mammary fat pad injection of red fluorescence protein (rfp)-expressing human breast cancer cell lines was recorded and quantified using a single lens reflex (slr) digital camera.

Results:

This simple system enabled the verification of successful tumor take and temporal quantification of tumor progression in mouse models.

Conclusion:

The macroscopic fluorescence imaging system represents an inexpensive and portable tool to facilitate non-invasive in situ cancer detection with the potential to monitor fluorescent tumor formation and investigation of the efficacy of potential cancer therapeutics.