Analysis of numerical aberrations in specific chromosomes by fluorescent in situ hybridization as a diagnostic tool in breast cancer

✍ Scribed by Daisuke Ichikawa; Naoya Hashimoto; Masakazu Hoshima; Toshiharu Yamaguchi; Kiyoshi Sawai; Yusuke Nakamura; Toshio Takahashi; Tatsuo Abe; Johji Inazawa

• Publisher: John Wiley and Sons
• Year: 1996
• Tongue: English
• Weight: 572 KB
• Volume: 77
• Category: Article
• ISSN: 0008-543X
• DOI: 10.1002/(sici)1097-0142(19960515)77:10<2064::aid-cncr15>3.0.co;2-t

✦ Synopsis

BACKGROUND. Biopsy by fine-needle aspiration has become a routine technique

for the diagnosis of a dominant breast mass. In this study, fluorescent in situ hybridization (FISH) analysis of interphase nuclei allowed the authors to detect genetic aberrations that are difficult to identify by conventional cytology.

METHODS.

To investigate ways of minimizing misdiagnosis of the cytology of breast tumors, and detecting genetic aberrations preoperatively, the authors performed FISH with specimens obtained by fine-needle aspiration biopsies of 106 primary breast tumors (78 primary breast cancers, 2 phyllodes tumors, and 26 benign breast tumors). Numerical chromosome aberrations were investigated using 3-color FISH performed with (perilcentromere-specific probes for chromosomes 1, 11, and 17.