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In-gel detection of L-amino acid oxidases based on the visualisation of hydrogen peroxide production

✍ Scribed by Jan Erik Rau; Ulrich Fischer


Book ID
116751075
Publisher
Elsevier Science
Year
2011
Tongue
English
Weight
177 KB
Volume
85
Category
Article
ISSN
0167-7012

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A simple procedure has been developed for the detection of L-amino acid oxidase, glutamine transaminase, pantetheinase, and acylase I in solution and on polyacrylamide gels. The method is based on the strong absorbance at 296 nm of some ketimine rings which can be directly produced by the enzymatic