Improving the expression of chimeric antibodies following homologous recombination in hybridoma cells
โ Scribed by Ralph Mocikat
- Book ID
- 114052175
- Publisher
- Elsevier Science
- Year
- 1999
- Tongue
- English
- Weight
- 103 KB
- Volume
- 225
- Category
- Article
- ISSN
- 0022-1759
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New type of plasmids named pEdHCG1 and pEdHC~:.were constructed. A combination of two plasmids containing the amplified gene product for the variable region of the antibody (Ab) heavy or light chains by the polymerase chain reaction (PCR) was co-transfected into COS cells to transiently express and
## Several analytical techniques were applied to obtain information about the stability of expression, yield and integrity of monoclonal antibodies (Mabs) produced by hybridoma cells in homogeneous continuous perfusion culture systems. An isotype-specific spot enzyme-linked immunosorbent assay (sp