Improving pH gradient cation-exchange chromatography of monoclonal antibodies by controlling ionic strength
β Scribed by Zhang, Liangyi; Patapoff, Thomas; Farnan, Dell; Zhang, Boyan
- Book ID
- 125453251
- Publisher
- Elsevier Science
- Year
- 2013
- Tongue
- English
- Weight
- 849 KB
- Volume
- 1272
- Category
- Article
- ISSN
- 1873-3778
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## Abstract Cation exchange chromatography separates adsorbed proteins by controlling the salt concentration or the mobile phase pH. This study examines the pHβbased method for binding and elution of monoclonal antibodies (MAbs). Five different clones with isoelectric points from 6 to 9 were evalua
## Abstract The characterization of three different mAb formulations of pharmaceutical interest is demonstrated using cation exchange polymethacrylateβbased monolithic columns as well as imaged CE (iCE). Elution of the mAbs from both a strong cation exchanger (ProSwift^TM^ SCXβ1S) and a weak cation