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Improvement of Yarrowia lipolytica lipase production by fed-batch fermentation

✍ Scribed by Patrick Fickers; Jacqueline Destain; Philippe Thonart

Publisher
John Wiley and Sons
Year
2009
Tongue
English
Weight
343 KB
Volume
49
Category
Article
ISSN
0233-111X

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✦ Synopsis

Abstract

Two different types of fed‐batch fermentation were investigated to improve production yields of the Lip2 extracellular lipase in Y. lipolytica mutant‐strain LgX64.81 grown in a 20l bioreactor. Compare to batch cultures, culture feeding with the complete medium led to a 2‐fold increased lipase production (2016 ± 76 U ml^–1^) whereas addition of a combination of glucose and olive oil led to a 3‐fold increase. The high level of lipase production obtained on glucose media with Y. lipolytica LgX64.81 could be related to its phenotype i.e. a lower sensibility to glucose catabolite repression due to a modification in the level of HXK1 expression. (© 2009 WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim)

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