Improvement of heterologous protein productivity using recombinant Yarrowia lipolytica and cyclic fed-batch process strategy
✍ Scribed by Ching Chuan Chang; Dewey D. Y. Ryu; Cheon Seok Park; Jeong-Yoon Kim
- Publisher
- John Wiley and Sons
- Year
- 1998
- Tongue
- English
- Weight
- 111 KB
- Volume
- 59
- Category
- Article
- ISSN
- 0006-3592
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✦ Synopsis
A cyclic fed-batch bioprocess is designed and a significant improvement of rice ␣-amylase productivity of recombinant Yarrowia lipolytica is illustrated. A bioprocess control strategy developed and reported here entails use of a genetically stable recombinant cloned for heterologous protein, use of optimized media for cell growth and enzyme production phases, and process control strategy enabling high cell-density culture and high ␣-amylase productivity. This process control can be achieved through maintaining a constant optimal specific cell growth rate at a predetermined value (i.e., 0.1 h -1 ), controlling medium feed rate commensurate with the cell growth rate, and maintaining a high cell-density culture (i.e., 60-70 g/L) for high productivity of cloned heterologous protein. The volumetric enzyme productivity (1,960 units/L и h) achieved from the cyclic fed-batch process was about 3-fold higher than that of the fedbatch culture process (630 units/L и h).