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Improvement of the method of Karnovsky and Roots for the histochemical demonstration of acetylcholinesterase

✍ Scribed by P. Kugler

Publisher
Springer
Year
1987
Tongue
English
Weight
555 KB
Volume
86
Category
Article
ISSN
1432-119X

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A histochemical method for the demonstra
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The "direct coloring" thiocholine method of Karnovsky and Roots (1964) for the demonstration of acetylcholinesterase (ACHE) activity was modified and adapted to the technique of semipermeable membranes. In this way it is possible to demonstrate histochemically both the bound as well as the soluble p

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In the original Karnovsky and Roots' method for the localization of acetylcholinesterase (AChE), thiocholine reduces the ferricyanide and cupric ions of this medium competitively, giving simultaneously cupric (Koelle's precipitate) as histochemical products. We modified the method in order to promot

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✍ Ms. Elizabeth K. Bjornskov; Dr. Forbes H. Norris Jr.; Dr. Janis Mower-Kuby πŸ“‚ Article πŸ“… 1982 πŸ› John Wiley and Sons 🌐 English βš– 475 KB

## Abstract Fluoresceinated alpha‐bungarotoxin (α‐BT) and the Pestronk‐Drachman neuromuscular stain were combined to demonstrate the acetylcholine receptor, the junctional acetylcholinesterase, and the nerve terminal on the same endplate. The method is reliable and rapid. Synapses in both normal mu