A successful procedure has been designed for the regeneration of plantlets from leaf sections of the self-pollinating species, Medicago suffruticosa. Callus growth was promoted by a 4-week culture period on liquid Kao's medium containing 4.9 #M benzyladenine and 4.5 #M 2,4-dichlorophenoxyacetic acid
Improvement of somatic embryogenesis inMedicago arborea
โ Scribed by Oscar Hita; Piedad Gallego; Nieves Villalobos; Itziar Lanas; Antonio Blazquez; Juan Pedro Martin; Javier Fernandez; Luisa Martin; Hilario Guerra
- Book ID
- 110406856
- Publisher
- Springer
- Year
- 2003
- Tongue
- English
- Weight
- 76 KB
- Volume
- 72
- Category
- Article
- ISSN
- 0167-6857
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Seventy-six cultivars of alfalfa (Medicago sativa L., M. faleata L. and M. varia Martyn) were tested in vitro for their capacity to produce callus and somatic embryos. A three-step media protocol was used to survey the response of the cotyledons and hypocotyl of each genotype while the epicotyl regi
A highly efficient system for direct somatic embryogenesis is described. Leaf sections originating from young trifoliate leaves of Medica~o fa[cata line 47/1-5 and Medicago saliva line No2/9R, directly produced embryos after cultivation in liquid BSIV induction medium. In comparison with indirect so