Improved synthesis of disaccharides with Escherichia coli β-galactosidase using bio-solvents derived from glycerol

Immobilisation of β-d-galactosidase from

Immobilisation of β-d-galactosidase from Escherichia coli on cellulose beads and its use for the synthesis of disaccharide derivatives

✍ Vladimír Kéry; Štefan Kučár; Mária Matulová; Jana Haplová 📂 Article 📅 1991 🏛 Elsevier Science 🌐 English ⚖ 318 KB

beta-D-Galactosidase, isolated from cloned E. coli, was immobilised on cellulose beads via oxidation with sodium periodate, activation by cyanuric chloride, or diazotisation. beta-D-Galactosidase immobilised via azo bonds showed the highest relative activity and thermostability, and was used for syn