✦ LIBER ✦

Immobilisation of β-d-galactosidase from Escherichia coli on cellulose beads and its use for the synthesis of disaccharide derivatives

✍ Scribed by Vladimír Kéry; Štefan Kučár; Mária Matulová; Jana Haplová

Publisher: Elsevier Science
Year: 1991
Tongue: English
Weight: 318 KB
Volume: 209
Category: Article
ISSN: 0008-6215
DOI: 10.1016/0008-6215(91)80146-e

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✦ Synopsis

beta-D-Galactosidase, isolated from cloned E. coli, was immobilised on cellulose beads via oxidation with sodium periodate, activation by cyanuric chloride, or diazotisation. beta-D-Galactosidase immobilised via azo bonds showed the highest relative activity and thermostability, and was used for synthesis of disaccharide methyl glycosides.