Improved staining method for determining the extent of thermal damage to cells

✍ Scribed by Margaret E. Sherwood; Thomas J. Flotte

• Publisher: John Wiley and Sons
• Year: 2007
• Tongue: English
• Weight: 169 KB
• Volume: 39
• Category: Article
• ISSN: 0196-8092
• DOI: 10.1002/lsm.20450

✦ Synopsis

Abstract

Background and Objective

Enzyme histochemical stains of frozen sections have been used by investigators to assess thermal damage. The assessment of thermal damage to cells in lipid‐rich tissues such as subcutaneous tissue and sebaceous glands can be difficult due to the quality of frozen sections of such tissues. The purpose of this study is to develop an improved method for this type of evaluation.

Study Design/Materials and Methods

Thick frozen sections of thermally damaged pig and human skin were stained for lactate dehydrogenase. The sections were fixed in formalin and processed for paraffin‐embedded sections.

Results

The sections showed well‐defined localization of the enzymatic deposits as well as preservation of the tissue architecture.

Conclusion

The paraffin‐embedded lactate dehydrogenase stained sections provide improved evaluation of thermally damaged tissues, particularly the lipid rich tissues. Lasers Surg. Med. 39:128–131, 2007. © 2007 Wiley‐Liss, Inc.