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Improved spectrophotometric determination of lactose in milk using flow-injection analysis

✍ Scribed by Dyer Narinesingh; Valerie A. Stoute; Gershwin Davis; Deenesh Persad; That T. Ngo

Publisher: Elsevier Science
Year: 1992
Tongue: English
Weight: 676 KB
Volume: 258
Category: Article
ISSN: 0003-2670
DOI: 10.1016/0003-2670(92)85207-m

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✦ Synopsis

Mdk samples were analysed spcctrophotometncally for their lactose content usmg flow-lwectlon analysis The methodology IS based on the carmme colour that develops (Amu = 540 nm) when lactose reacts with alkahne methylamme (pH 12 7) Lactose concentrations as low as 0 6 mg ml-' can be readily detected and up to 40 samples can be analysed per hour Excellent correlations (r > 0 999) are obtamed when the results arc compared wrth the batch and hqmd chromatographx methods for lactose determmatlon Interference from maltose, If present, should be ebmmated by pretreatment wth maltase &WW& Spectrophotometry, Flow system, Lactose, Mdk

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