Improved resolution in three-dimensional constant-time triple resonance NMR spectroscopy of proteins
β Scribed by Arthur G. Palmer; Wayne J. Fairbrother; John Cavanagh; Peter E. Wright; Mark Rance
- Book ID
- 105491911
- Publisher
- Springer Netherlands
- Year
- 1992
- Tongue
- English
- Weight
- 337 KB
- Volume
- 2
- Category
- Article
- ISSN
- 0925-2738
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Nonlinear sampling along the constant-time dimension is applied to the constant-time HNCO spectrum of the dimerization domain of Gal4. Nonlinear sampling was used for the nitrogen dimension, while the carbon and proton dimensions were sampled linearly. A conventional ct-HNCO spectrum is compared wit
Triple-resonance NMR experiments are nearly essential for performing backbone assignments of proteins larger than approximately 15 kDa. Our work extends the double constant-time (2CT) evolution scheme to triple-resonance 3D and 4D experiments. The modifications needed to accomplish 2CT evolution in