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Three-dimensional deuterium-carbon correlation experiments for high-resolution solid-state MAS NMR spectroscopy of large proteins

✍ Scribed by Daniela Lalli; Paul Schanda; Anup Chowdhury; Joren Retel; Matthias Hiller; Victoria A. Higman; Lieselotte Handel; Vipin Agarwal; Bernd Reif; Barth van Rossum; Ümit Akbey; Hartmut Oschkinat


Publisher
Springer Netherlands
Year
2011
Tongue
English
Weight
530 KB
Volume
51
Category
Article
ISSN
0925-2738

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Three-Dimensional Solid-State NMR Spectr
✍ Francesca M Marassi; Che Ma; Jennifer J Gesell; Stanley J Opella 📂 Article 📅 2000 🏛 Elsevier Science 🌐 English ⚖ 188 KB

Uniformly 15 N-labeled samples of membrane proteins with helices aligned parallel to the membrane surface give two-dimensional PISEMA spectra that are highly overlapped due to limited dispersions of 1 H-15 N dipolar coupling and 15 N chemical shift frequencies. However, resolution is greatly improve