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Improved protoplast culture and regeneration of shoots in pea (Pisum sativumL.)

✍ Scribed by Johanna Puonti-Kaerlas; Tage Eriksson


Publisher
Springer
Year
1988
Tongue
English
Weight
737 KB
Volume
7
Category
Article
ISSN
0721-7714

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A procedure has been developed which allows high frequency adventitious shoot regeneration from immature cotyledons of pea. Prolific shoot regeneration occurred following an initial callus growth on a Murashige and Skoog (MS) medium containing 0.5 mg/l 6-benzylaminopurine (BAP) and 4 mg/l Ξ±-naphthal

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Complementary DNA clones and a corresponding nuclear gene (lpcr) encoding the NADPH-dependent protochlorophyllide oxidoreductase (pchlide reductase, EC 1.6.99.1) have been characterized from pea (Pisum sativum L.). The pea lpcr gene encodes a 43 118 Da precursor polypeptide comprised of a transit pe