DNA variations in regenerated plants of pea (Pisum sativumL.)
β Scribed by E. Cecchini; L. Natali; A. Cavallini; M. Durante
- Publisher
- Springer
- Year
- 1992
- Tongue
- English
- Weight
- 635 KB
- Volume
- 84-84
- Category
- Article
- ISSN
- 0040-5752
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The percentage of mitochondrial DNA (mtDNA) present in total DNA isolated from pea tissues was determined using labeled mtDNA in reassociation kinetics reactions. Embryos contained the highest level of mtDNA, equal to 1.5% of total DNA. This value decreased in light-and dark-grown shoots and leaves,
A procedure has been developed which allows high frequency adventitious shoot regeneration from immature cotyledons of pea. Prolific shoot regeneration occurred following an initial callus growth on a Murashige and Skoog (MS) medium containing 0.5 mg/l 6-benzylaminopurine (BAP) and 4 mg/l Ξ±-naphthal